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recombinant mouse mer protein  (MedChemExpress)


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    MedChemExpress recombinant mouse mer protein
    Recombinant Mouse Mer Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+mer+protein/OF-1/pmc12570178-54-0-4
    Average 99 stars, based on 667 article reviews
    recombinant mouse mer protein - by Bioz Stars, 2026-09
    99/100 stars

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    Expi293F based mammalian expression system produced soluble trimeric prefusion MERS-CoV Spike. (a) schematic representation of color-coded expression cassette used in mammalian (pcDNA3.1 (1)) expression vector; blue box represents signal peptide sequence at N-terminal domain for mammalian expression; light-green box represents human-codon-optimized gene encoding M-CoV-S sequences from 16–1291 amino acids taken from England 1 strain; and at C-terminal domain dark-green box represents T4 fibritin trimerization domain and pink box for 8×His-tag. (b)12% reducing SDS-PAGE of purified M-CoV-S (Lane1: Marker, Lane 2: M-CoV-S). (c) Western blot image of inhouse M-CoV-S and commercial MERS-RBD (#10621-CV, R&D systems) probed with anti-M-CoV-S mouse polyclonal serum (Lane 1, 4: Marker, Lane 2: In-house M-CoV-S, Lane 3: recombinant MERS-RBD). (d) Blue native-PAGE image of purified M-CoV-S protein. Lane 1: native Marker, Lane 2: native M-CoV-S. (e) Purified M-CoV-S protein was subjected to de-glycosylation by using PNGase F enzyme (Lane 1: Marker, Lane 2: PNGase treated M-CoV-S, Lane 3: untreated M-CoV-S).

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Characterization and immunogenicity assessment of MERS-CoV pre-fusion spike trimeric oligomers as vaccine immunogen

    doi: 10.1080/21645515.2024.2351664

    Figure Lengend Snippet: Expi293F based mammalian expression system produced soluble trimeric prefusion MERS-CoV Spike. (a) schematic representation of color-coded expression cassette used in mammalian (pcDNA3.1 (1)) expression vector; blue box represents signal peptide sequence at N-terminal domain for mammalian expression; light-green box represents human-codon-optimized gene encoding M-CoV-S sequences from 16–1291 amino acids taken from England 1 strain; and at C-terminal domain dark-green box represents T4 fibritin trimerization domain and pink box for 8×His-tag. (b)12% reducing SDS-PAGE of purified M-CoV-S (Lane1: Marker, Lane 2: M-CoV-S). (c) Western blot image of inhouse M-CoV-S and commercial MERS-RBD (#10621-CV, R&D systems) probed with anti-M-CoV-S mouse polyclonal serum (Lane 1, 4: Marker, Lane 2: In-house M-CoV-S, Lane 3: recombinant MERS-RBD). (d) Blue native-PAGE image of purified M-CoV-S protein. Lane 1: native Marker, Lane 2: native M-CoV-S. (e) Purified M-CoV-S protein was subjected to de-glycosylation by using PNGase F enzyme (Lane 1: Marker, Lane 2: PNGase treated M-CoV-S, Lane 3: untreated M-CoV-S).

    Article Snippet: The membrane was blocked in blocking buffer for 2 hours at 37°C followed by incubation with anti-MERS-CoV-S mouse polyclonal sera (1:5000) and commercial MERS-RBD (#10621-CV, R&D Systems) (1:1000) at 4°C for overnight.

    Techniques: Expressing, Produced, Plasmid Preparation, Sequencing, SDS Page, Purification, Marker, Western Blot, Recombinant, Blue Native PAGE, Glycoproteomics